the investigation of heat shock protein (hsp70) expression change in human brain asterocytoma tumor
نویسندگان
چکیده
abstract background: asterocytoma is the most common primary human brain tumor which has the most lethal hazard among tumors in human central nervous system. heat shock proteins play an active role in cancer cells pathways through their effects on tumor cell proliferation, tumor differentiation, malignancy, metastasis, and cell death. hsp 70 is a 70 kd protein that its expression change has been reported in various kinds of cancers. here a proteomic activity for molecular diagnosis of asterocytomas tumors is designed. methods: proteins of tumor and normal brain tissues were extracted and then evaluated by bradford test. in this study, the proteins were separated by 2dg electrophoresis method, and then the spots were analyzed and compared using statistical data and specific software, after providing 3d images of spots alteration. spots were identified by isoelectric point (pi), molecular weights and data banks. results: as a result, the 2d gel showed totally 800 spots. hsp 70 is one of down regulated proteins. conclusion: as conclusion, alteration of hsp 70 expression was detected in asterocytomas which has an important role in tumor survival and development, response to cell stress and induces apoptosis in cells. three kinds of hsp 70 are known in different cancers: hsp 70-1 , hsp70 -2 and hsp 70-8 which affect limitation of apoptosis system in cancer cells.
منابع مشابه
The human heat shock protein hsp70 interacts with HSF, the transcription factor that regulates heat shock gene expression.
Transcriptional regulation of the human hsp70 gene in response to heat shock and other forms of physiological stress occurs through the activation of heat shock transcription factor (HSF). Exposure of cells to a heat shock temperature of 42 degrees C results in transient activation of HSF; its DNA-binding activity increases rapidly, plateaus, and attenuates, during which the intracellular level...
متن کاملStructure and expression of the human gene encoding major heat shock protein HSP70.
We have cloned a human gene encoding the 70,000-dalton heat shock protein (HSP70) from a human genomic library, using the Drosophila HSP70 gene as a heterologous hybridization probe. The human recombinant clone hybridized to a 2.6-kilobase polyadenylated mRNA from HeLa cells exposed to 43 degrees C for 2 h. The 2.6-kilobase mRNA was shown to direct the translation in vitro of a 70,000-dalton pr...
متن کاملHeat shock protein expression in human conjunctiva.
PURPOSE To examine the distribution of human heat shock proteins (HSPs) HSP90, inducible HSP70 (iHSP70), constitutive and inducible HSP70 (cHSP70), HSP65, and human HSP27 in conjunctival biopsy specimens of ocular cicatricial pemphigoid (OCP), atopic keratoconjunctivitis, and healthy persons with cataract. METHODS Using an immunoperoxidase technique, conjunctival biopsy specimens from ten pat...
متن کاملLocalization of the heat-shock protein Hsp70 to the synapse following hyperthermic stress in the brain.
Heat-shock proteins are induced in response to cellular stress. Although heat-shock proteins are known to function in repair and protective mechanisms, their relationship to critical neural processes, such as synaptic function, has received little attention. Here we investigate whether the major heat-shock protein Hsp70 localizes to the synapse following a physiologically relevant increase in t...
متن کاملmolecular, evolution and stratification study of the heat shock protein 70 (hsp70) genes family in cattle
heat shock proteins (hsps), are highly important due to their association with such economically important traits in animals as resistance to temperature shock and mastitis. in the current study, to get a comprehensive information on molecular structure and evolution of the hsp70s, and the available hsp70 sequences of the cattle and other animals taken from ncbi and aligned together. then, nucl...
متن کاملRole of heat shock protein HSP70-2 in spermatogenesis.
The HSP70 heat-shock proteins are molecular chaperones that assist other proteins in their folding, transport and assembly into complexes. Most of these proteins are either constitutively expressed or their expression is induced by heat shock and other stresses. However, two members of the Hsp70 family (HSP70-2 and HSC70T in mice) are regulated developmentally and expressed specifically in sper...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of cancer preventionجلد ۶، شماره ۰۳، صفحات ۶-۱۱
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023